b cereus atcc 14579 spans 22 kb  (ATCC)

Journal: mSphere

Article Title: Beyond commensalism: genomic insights into micrococcin P1-producing Staphylococcus chromogenes

doi: 10.1128/msphere.00733-25

Figure Lengend Snippet: Comparative analysis of micrococcin P1 BGCs across diverse bacterial species. The gene cluster identified in Staphylococcus chromogenes strains 4S77 and 4S90 is shown alongside homologous clusters from Bacillus cereus ATCC 14579 (GenBank accession no. CP034551 ), S. hominis M1 (this study), S. hominis S34-1 ( CP040733 ), S. equorum KAVA ( MW633237 ), S. capitis Sc1516939 ( CP145210 ), S. aureus UP_1591 ( CP047810 ), S. agnetis 4244 ( JABULG020000000 ), and Macrococcus caseolyticus 115 ( KM613043 ). The sequence from Mammaliicoccus sciuri IMDO-S72 was not available and could not be included. Clusters were identified on either plasmids or chromosomes. Gene functions are color-coded as indicated in the legend. The core biosynthetic genes ( tclE–tclQ ) exhibit strong conservation across Staphylococcus species, with sequence similarity illustrated by shaded gray connectors reflecting amino acid identity. ND, not determined.

Article Snippet: In a more complex arrangement, the chromosomal MP1 cluster of B. cereus ATCC 14579 spans 22 kb and includes 24 genes, among them four precursor peptide genes ( tclE, tclF, tclG, and tclH ), two L11-type immunity genes ( tclQ and tclT ), and several additional modification enzymes ( tclD, tclO, and tclV ) not found in staphylococcal-type clusters ( ).

Techniques: Sequencing

Journal: mSphere

Article Title: Beyond commensalism: genomic insights into micrococcin P1-producing Staphylococcus chromogenes

doi: 10.1128/msphere.00733-25

Figure Lengend Snippet: Sequence conservation, structure, and functional validation of micrococcin P1 (MP1) from S. chromogenes strains 4S77 and 4S90. ( A ) Alignment of the precursor peptide encoded by tclE from the MP1 cluster in S. chromogenes strains 4S77 and 4S90 with homologous sequences from Bacillus cereus ATCC 14579 ( CP034551 ), S. hominis M1 (this study), S. hominis S34-1 ( CP040733 ), S. equorum KAVA ( MW633237 ), S. capitis Sc1516939 ( CP145210 ), S. aureus UP_1591 ( CP047810 ), S. agnetis 4244 ( JABULG020000000 ), and Macrococcus caseolyticus 115 ( KM613043 ). The sequence from Mammaliicoccus sciuri IMDO-S72 was not available and could not be included. The red arrow marks the predicted cleavage site between the leader and core regions, with the red box indicating the conserved 14-residue core peptide subject to post-translational modification; ( B ) Schematic representation of post-translational modifications on the 14-residue core peptide leading to mature MP1. Color legend indicates the different modification types. Adapted from Wieland Brown et al . ; ( C ) RP-HPLC chromatogram of the purified compound from S. chromogenes strains 4S77 and 4S90, showing a dominant peak at the expected retention time for MP1. The inset shows the inhibition zone produced by this purified sample (2.5 µg) against S. aureus JE2.

Article Snippet: In a more complex arrangement, the chromosomal MP1 cluster of B. cereus ATCC 14579 spans 22 kb and includes 24 genes, among them four precursor peptide genes ( tclE, tclF, tclG, and tclH ), two L11-type immunity genes ( tclQ and tclT ), and several additional modification enzymes ( tclD, tclO, and tclV ) not found in staphylococcal-type clusters ( ).

Techniques: Sequencing, Functional Assay, Biomarker Discovery, Residue, Modification, Purification, Inhibition, Produced